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  1. Hi Doc, I think there is something important to know about CBD isolate. One, Endoca is providing suppositories that have 50mg of CBD and perhaps one other form of it, maybe CBDG, not sure, but here is the importance of the fact that they are making and selling them, a handy person will of course make their own. I'm pretty sue this would be important for someone facing prostate cancer as well, I have heard a few people claiming they beat it with CBD alone and I know the THC people will have an issue with that. There was one older gentleman from the U.K. who had it metastasized to his pubic bone… and all he could get was CBD, his story is on cannabis health radio channel on YT.
    BMC Complement Altern Med. 2016 Sep 1;16(1):335. doi: 10.1186/s12906-016-1280-0.
    Cannabidiol rather than Cannabis sativa extracts inhibit cell growth and induce apoptosis in cervical cancer cells.
    Lukhele ST1, Motadi LR2.
    Author information
    Abstract
    BACKGROUND:
    Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year. Different therapeutic regimens have been suggested in various regions of Africa, however, over a quarter of a million women die of cervical cancer, annually. This makes it the most lethal cancer amongst black women and calls for urgent therapeutic strategies. In this study we compare the anti-proliferative effects of crude extract of Cannabis sativa and its main compound cannabidiol on different cervical cancer cell lines.

    METHODS:
    To achieve our aim, phytochemical screening, MTT assay, cell growth analysis, flow cytometry, morphology analysis, Western blot, caspase 3/7 assay, and ATP measurement assay were conducted.

    RESULTS:
    Results obtained indicate that both cannabidiol and Cannabis sativa extracts were able to halt cell proliferation in all cell lines at varying concentrations. They further revealed that apoptosis was induced by cannabidiol as shown by increased subG0/G1 and apoptosis through annexin V. Apoptosis was confirmed by overexpression of p53, caspase 3 and bax. Apoptosis induction was further confirmed by morphological changes, an increase in Caspase 3/7 and a decrease in the ATP levels.

    CONCLUSIONS:
    In conclusion, these data suggest that cannabidiol rather than Cannabis sativa crude extracts prevent cell growth and induce cell death in cervical cancer cell lines.

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